Littleton Washington was born in Washington D.C. on November 3, 1825, the son of Lund Washington, whose forebears were cousins of the family of the first president. He enrolled with the class of 1845 at Dickinson College in Carlisle, Pennsylvania and, while there, was an active student, gaining election to the Belles Lettres Society. He was forced, though, to withdraw from the College due to family financial difficulties. He found gainful employment instead as a clerk in the U.S. Treasury.

Marcus Junius Parrott was born on October 29, 1828 in Hamburg, South Carolina, the son of a wealthy Quaker family. His parents left the South when he was a young boy and he grew up in Dayton, Ohio. He was prepared at the Dayton Academy, and went on to study at Ohio Wesleyan University. In December, 1847, Parrott was expelled from Ohio Wesleyan over a clash with his Greek instructor and his refusal to sign a pledge to respect that faculty member.

Horatio Collins King was born on December 22, 1837 in Portland, Maine, to Horatio and Anne Collins King. The elder King served as postmaster general in the cabinet of James Buchanan. Horatio Collins King was prepared at Emory and Henry College. In 1854 he entered Dickinson College, where his uncle Charles Collins was president. While there, he was a member of Phi Kappa Sigma and the Union Philosophical Society. He earned a bachelor's degree in 1858; King was later inducted into Phi Beta Kappa when a chapter was established at Dickinson in 1886.

John A. Inglis was born in Baltimore, Maryland on August 26, 1813, the son of well known Presbyterian minister James Inglis, then pastor of the First Presbyterian Church in the city. He entered Dickinson College and graduated with the class of 1829 and then taught school for a time in Carlisle, eventually studying law and relocating to South Carolina.

John H. Grabill was born to Ephraim and Caroline Grabill in Mount Jackson, Virginia on March 8, 1839. He prepared at the Woodstock and Harrisburg Academies and entered Dickinson College in Carlisle, Pennsylvania in 1858 with the class of 1860. While at the College, he became a member of the Phi Kappa Sigma fraternity and was elected to the Union Philosophical Society. He graduated with his class and returned to the Shenandoah Valley.